تنوع ژنتيکي در توالی ناحیه ITS ژنوم ريبوزومي هسته اي (RDNA) در بین توکسوکارای سگ و گربه مناطق مختلف ايران

عنوان انگليسی

GENETIC VARIATION IN THE SEQUENCES OF THE INTERNAL TRANSCRIBED SPACERS (ITS) OF NUCLEAR RIBOSOMAL DNA (RDNA) AMONG AND WITHIN TOXOCARA NEMATODE OF DOGS AND CATS FROM DIFFERENT AREAS IN IRAN

خلاصه انگلیسی

Toxocara canis and Toxocara cati are ascaridoid nematodes of dogs and cats. The objectives of the present study were to identify the sequence variation of the internal transcribed spacer (ITS) region within and among isolates of T. canis and T. cati from dogs and cats in Iran. Genomic DNA was extracted from 23 isolates of T. canis and 35 isolates of T. cati collected from dogs and cats in different geographical areas of Iran. ITS region in nuclear ribosomal DNA was PCRamplified. The PCR product of 5 isolates of T. canis and 9 isolates of T. cati were sequenced. The sequences were aligned using the BioEdit software and compared with published sequences in GenBank. Phylogenetic analysis was performed using Mega 5.0 software and Maximum likelihood method. The amplicons of about 1000 and 1100 bp were successfully produced for T. canis and T. cati, respectively. Pairwise comparison of the sequences did not show any differences in nucleotide sequences within T. canis isolates. Likewise, sequences of T. cati were identical and exhibiting 100% homology. However, 12% inter-species sequence difference was found between the isolates of T. canis and T. cati.Regarding to no intra-species variation and a significant inter-species variation in ITS region among T. canis and T. cati isolates, it is concluded that the region can be used for identification and differentiation of Toxocara species.

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