title

تنوع ژنتيکي در توالی ناحیه ITS ژنوم ريبوزومي هسته اي (RDNA) در بین توکسوکارای سگ و گربه مناطق مختلف ايران

میکائیلی, فتانه and میرهندی, حسین and محبعلی, مهدی and حسینی, مصطفی and زارعی, ذبیح الله and حیدری, زهرا and کیا, عشرت بیگم (1394) تنوع ژنتيکي در توالی ناحیه ITS ژنوم ريبوزومي هسته اي (RDNA) در بین توکسوکارای سگ و گربه مناطق مختلف ايران. در: 2nd International and 9th National Congress of Parasitology and Parasitic Diseases of Iran, May 20-22 , 2015, Guilan – Iran.

[img]
Preview
Text - Published Version
128kB
[img]
Preview
Image
79kB

Title

GENETIC VARIATION IN THE SEQUENCES OF THE INTERNAL TRANSCRIBED SPACERS (ITS) OF NUCLEAR RIBOSOMAL DNA (RDNA) AMONG AND WITHIN TOXOCARA NEMATODE OF DOGS AND CATS FROM DIFFERENT AREAS IN IRAN

English Abstract

Toxocara canis and Toxocara cati are ascaridoid nematodes of dogs and cats. The objectives of the present study were to identify the sequence variation of the internal transcribed spacer (ITS) region within and among isolates of T. canis and T. cati from dogs and cats in Iran. Genomic DNA was extracted from 23 isolates of T. canis and 35 isolates of T. cati collected from dogs and cats in different geographical areas of Iran. ITS region in nuclear ribosomal DNA was PCRamplified. The PCR product of 5 isolates of T. canis and 9 isolates of T. cati were sequenced. The sequences were aligned using the BioEdit software and compared with published sequences in GenBank. Phylogenetic analysis was performed using Mega 5.0 software and Maximum likelihood method. The amplicons of about 1000 and 1100 bp were successfully produced for T. canis and T. cati, respectively. Pairwise comparison of the sequences did not show any differences in nucleotide sequences within T. canis isolates. Likewise, sequences of T. cati were identical and exhibiting 100% homology. However, 12% inter-species sequence difference was found between the isolates of T. canis and T. cati.Regarding to no intra-species variation and a significant inter-species variation in ITS region among T. canis and T. cati isolates, it is concluded that the region can be used for identification and differentiation of Toxocara species.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :فتانه میکائیلی
نویسنده :حسین میرهندی
نویسنده :مهدی محبعلی
نویسنده :مصطفی حسینی
نویسنده :ذبیح الله زارعی
نویسنده :زهرا حیدری
نویسنده :عشرت بیگم کیا
کلیدواژه ها (انگلیسی):genetic variation, Toxocara canis, Toxocara cati, ITS Iran
Subjects:QX Parasitology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:9081
Deposited By: خانم زهرا حیدری سورشجانی
Deposited On:06 May 1398 13:27
Last Modified:06 May 1398 13:35

Repository Staff Only: item control page

Document Downloads

More statistics for this item...