title

مطالعه مقدماتی مولکولی ایزوله های اکینوکوکوس گرانولوزوس در چابهار استان سیستان و بلوچستان با استفاده از ژن cox1

اعتمادی, سودابه and حیدری, سودابه and حیدری, زهرا and مومنی, محمد and سرانی علی آبادی, پروانه and کامران رشانی, بهاره and کیا, عشرت بیگم (1394) مطالعه مقدماتی مولکولی ایزوله های اکینوکوکوس گرانولوزوس در چابهار استان سیستان و بلوچستان با استفاده از ژن cox1. در: 2nd International and 9th National Congress of Parasitology and Parasitic Diseases of Iran, May 20-22, 2015, Guilan- Iran.

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Title

PRELIMINALRY STUDY ON MOLECULAR CHARACTERIZATION OF ECHINOCOCCUS GRANULOSUS ISOLATES IN CHABAHAR SISTAN AND ALUCHESTAN PROVINCE IRAN BY COX1 GENE

English Abstract

Echinococcus granulosus is the causal agent of cystic hydatid disease in man and animals. The aim of this study was to characterize the isolates of E. granulosus from sheep and cattel by sequence analysis of cytochrome C oxidase subunit I (COX1) mitochondrial partial gene. Martial & Methods: Two isolates of hydatid cysts one from liver of sheep and other one from lung of cattel were collected from an abbatoir in Chabahar Sistan and Baluchestan Province Iran in 2014. The isolates were preserved in ethanol alchohol and transported to the Dept. of Medical Parasitology and Mycology School of Public Health Tehran University of Medical Sciences. DNA was extracted using a DNA kit from germinal layer and then the PCR amplification was performed for mitochondrial cox1 gene. The PCR products were sequenced after purification. The nucleotide sequences were edited by Chormas 2.4 software and aligned by BLAST. Then they were compared to those in the GenBank database. Result: Both E. granulosus isolates were determined as G1 strain. Their sequences had 99% homology with accession numbers KM 100573 and JQ250814 in GenBank.Similar to other parts of Iran, this preliminary study verifies the G1 genotype of E. granulosus as the probable dominant genotype in the study area.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :سودابه اعتمادی
نویسنده :زهرا حیدری
کلیدواژه ها (انگلیسی):Echinococcus granulosus, COX1 G1, genotype, Iran
Subjects:QX Parasitology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:9083
Deposited By: خانم زهرا حیدری سورشجانی
Deposited On:26 Jul 1397 12:52
Last Modified:26 Jul 1397 12:52

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