بررسی تنیا کولیس گوسفند به وسيله ژنوم میتوکندریال(COX1) در چابهار،استان زاهدان

عنوان انگليسی

TENUICOLLIS FROM SHEEP BY SEQUENCING OF MITOCHONDRIAL CYTOCHROME C OXIDASE SUBUNIT 1 (COX1) GENE IN CHABAHAR, ZAHEDAN PROVINCE

خلاصه انگلیسی

Cysticercus tenuicollis is the larval stage of the canine tapeworm Taenia hydatigena, which is prevalent in domestic and wild ruminants. T. hydatigena is one of the most prevalent cestodes in dogs in Iran. Metacestodes are in liver of intermediate host. The aim of this study was to provide molecular characterization of the sheep isolates of C. tenuicollis by PCR amplification partial sequencing of COX1 gene. C. tenuicollis bladders were collected from liver of sheep during routine veterinary inspection in abattoir of Chabahar, Zahedan province, Iran. Two samples were transported to Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences. Following DNA extraction, fragment of cytochrome C oxidase 1 (COX1) gene was amplified by PCR. After sequencing of amplicons, they were edited and aligned, using Chromas software. Sequences analysis was undertaken by BLAST algorithms and databases from GenBank. Result: The amplified fragment size was approximately 446 bp. Partial sequence of COX1 gene was corresponding with C. tenuicollis. The isolates were different with each other in one nucleotide.According to the alignment results; two isolates had 99% homology with C. enuicollis in GenBank with accession number DQ995656 and AB792722, respectively. In order to study probable difference between isolates in the study area, sequencing of more isolates from different hosts is needed.

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