مشخصات مولكولي تريكوسترنژيلوس کولوبریفورمیس و تریکوسترونژیلوس ويترينوس جدا شده از انسان در استان گيلان: اولین گزارش در ايران

عنوان انگليسی

MOLECULAR CHARACTERIZATION OF TRICHOSTRONGYLUS COLUBRIFORMIS AND T.VITRINUS ISOLATED FROM HUMAN IN GUILAN PrOVINCE: THE FIRST REPORT IN IRAN

خلاصه انگلیسی

Trichostrongyliasis, the infection caused by the different species of the Genus Trichostrongylus, has a worldwide distribution in ruminants. Up to the moment, more the 30 species havebeen recognized to infect mammals of which 10 species reported from humans. In Iran, 9 species of the genus Trichostrongylushave reported from livestock, mainly from centraland southwestern parts of the country. Interestingly, all thesespecies have also been obtained from humans. Close contactwith herbivorous animals and use of fresh livestock manure as fertilizer are the main cause of human infections. The fecalmaterials of a symptomatic patient from Langroud district of Guilan province, who had been diagnosed to be infected with Trichostrongylus spp., were collected after treatment with combination of Mebendazole and Albendazole. The male worms were obtained from the fecal materials identified as T. colubriformis and T. vitrinus by morphological characteristics. For definitive identification of the parasites a Polymerase Chain Reaction (PCR), specific for rDNA ITS2 was performed using the primer set of NC1: 5-ACGTCTGGTTCAGGGTTGTT-3) and NC2: 5- TTAGTTTCTTTTCCTCCGCT-3) (Chilton, 2004). In present study, amplicon of about 328 base pair (bp) was successfully produced by PCR for ITS2 region and using the BLAST system, Trichostrongylus isolates were identified as T. colubriformis and T. vitrinus. Sequences of these two human isolates weredeposited in GenBank database (accession numbers:KF826913and KF872228, respectively).This study is the firstmolecular evidence of human isolates of T. colubriformis and T.vitrinus in Iran. The ITS2 sequence of Iranian T. vitrinus wasidentical to ITS2 sequences of T. vitrinus from New Zealand(Accession No. KC998731) and United Kingdom (Accession No. AY439027) and that of T. colubriformis was identical to isolates of Iran (animal isolate), Laos, Australia and Malaysia found in GenBank.

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