توصیف مورفولوژیکی و مولکولی گونه های تریکوسترونژیلوس در گوسفندان مشگین شهر، استان اردبیل، ایران

عنوان انگليسی

MORPHOLOGICAL AND MOLECULAR DESCRIBTION OF TRICHOSTRONGYLUS SPECIES IN MESHKINSHAR SHEEP, ARDABIL PROVINCE, IRAN

خلاصه انگلیسی

Nematodes of family Trichostrongylidae live in abomasum and beginning of small intestines of ruminants and herbivores.They can be pathogenic in humans as well. In this study, morphological and molecular characterization of Trichostrongylus isolated from sheep in Meshkinshar is investigated. In 2012, the contents of abomasum and small intestine of sheep, slaughtered in a traditional abbotoir in Meshkinshahr were examined and Trichostrongylus nematodes were collected. Morphological characteristics of nematodes were studied after clearing and staining, using a calibrated microscope equipped with a drawing tube. Genomic DNA was extracted and ITS2-rDNA region was amplified by PCR. PCR products were sequenced and compared with sequences in the GenBank database. Three species including Trichostrongylus axei, Trichostrongylus columbiformis and Trichostrongylus vitrinus were identified. All species formed similar band on gel electrophoresis.Analysis of sequences revealed the presence of T. axei (KJ755059.1), T. colubriformis (KJ755060.1) and T. vitrinus (KJ755061.1), all with 100% homology with relevant Sequences in GenBank.Species of T. axei, T. colubriformis and T. vitrinus are comom in sheep in Meshkinshahr, and the dominant species is T. vitrinus.

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