title

توصیف مورفولوژیکی و مولکولی گونه های تریکوسترونژیلوس در گوسفندان مشگین شهر، استان اردبیل، ایران

حیدری, زهرا and موبدی, ایرج and زارعی, ذبیح الله and شریف دینی, میثم and کیا, عشرت بیگم (1394) توصیف مورفولوژیکی و مولکولی گونه های تریکوسترونژیلوس در گوسفندان مشگین شهر، استان اردبیل، ایران. در: 2nd International and 9th National Congress of Parasitology and Parasitic Diseases of Iran, May 20- 22, 2015, Guilan- Iran.

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Title

MORPHOLOGICAL AND MOLECULAR DESCRIBTION OF TRICHOSTRONGYLUS SPECIES IN MESHKINSHAR SHEEP, ARDABIL PROVINCE, IRAN

English Abstract

Nematodes of family Trichostrongylidae live in abomasum and beginning of small intestines of ruminants and herbivores.They can be pathogenic in humans as well. In this study, morphological and molecular characterization of Trichostrongylus isolated from sheep in Meshkinshar is investigated. In 2012, the contents of abomasum and small intestine of sheep, slaughtered in a traditional abbotoir in Meshkinshahr were examined and Trichostrongylus nematodes were collected. Morphological characteristics of nematodes were studied after clearing and staining, using a calibrated microscope equipped with a drawing tube. Genomic DNA was extracted and ITS2-rDNA region was amplified by PCR. PCR products were sequenced and compared with sequences in the GenBank database. Three species including Trichostrongylus axei, Trichostrongylus columbiformis and Trichostrongylus vitrinus were identified. All species formed similar band on gel electrophoresis.Analysis of sequences revealed the presence of T. axei (KJ755059.1), T. colubriformis (KJ755060.1) and T. vitrinus (KJ755061.1), all with 100% homology with relevant Sequences in GenBank.Species of T. axei, T. colubriformis and T. vitrinus are comom in sheep in Meshkinshahr, and the dominant species is T. vitrinus.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :زهرا حیدری
نویسنده :عشرت بیگم کیا
کلیدواژه ها (انگلیسی):Trichostrongylus, sheep, ITS-2, Iran
Subjects:QX Parasitology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:9087
Deposited By: خانم زهرا حیدری سورشجانی
Deposited On:21 Jul 1397 15:52
Last Modified:21 Jul 1397 15:52

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