مهار گلوکز 6-فسفات دهيدروژناز از هپاتوسيت ها در مقابل سميت ناشي از آلومينيوم فسفيد محافظت می کند.

سلیمی, احمد and پاییزی, مریم and سادات یوسف ثانی, بهاره and شادنیا, شاهین and حسنیان مقدم, حسین and زمانی, نسیم and پوراحمد, جلال (1396) مهار گلوکز 6-فسفات دهيدروژناز از هپاتوسيت ها در مقابل سميت ناشي از آلومينيوم فسفيد محافظت می کند. Pesticide Biochemistry and Physiology ــ 143 . pp. 141-146. شاپا 0048-3575


Official URL: http://www.sciencedirect.com/science/article/pii/S...


Inhibition of glucose-6-phosphate dehydrogenase protects hepatocytes from aluminum phosphide-induced toxicity

English Abstract

Aluminum phosphide (AlP) poisoning is a severe toxicity with 30–70% mortality rate. However, several case reports presented AlP-poisoned patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency and extensive hemolysis who survived the toxicity. This brought to our mind that maybe G6PD deficiency could protect the patients from severe fatal poisoning by this pesticide. In this research, we investigated the protective effect of 6-aminonicotinamide (6-AN)- as a well-established inhibitor of the NADP+- dependent enzyme 6-phosphogluconate dehydrogenase- on isolated rat hepatocytes in AlP poisoning. Hepatocytes were isolated by collagenase perfusion method and incubated into three different flasks: control, AlP, and 6-AN + ALP. Cellar parameters such as cell viability, reactive oxygen species (ROS) formation, mitochondria membrane potential collapse (MMP), lysosomal integrity, content of reduced (GSH) and oxidized glutathione (GSSG) and lipid peroxidation were assayed at intervals. All analyzed cellular parameters significantly decreased in the third group (6-AN+ AlP) compared to the second group (AlP), showing the fact that G6PD deficiency induced by 6-AN had a significant protective effect on the hepatocytes. It was concluded that G6PD deficiency significantly reduced the hepatotoxicity of AlP. Future drugs with the power to induce such deficiency may be promising in treatment of AlP poisoning.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :احمد سلیمی
نویسنده :مریم پاییزی
نویسنده :بهاره سادات یوسف ثانی
نویسنده :شاهین شادنیا
نویسنده :حسین حسنیان مقدم
نویسنده مسئول :نسیم زمانی
نویسنده مسئول :جلال پوراحمد
Additional Information:IF: 3.44 Indexed in: ISI, PubMed/Medline, Scopus
کلیدواژه ها (انگلیسی):Aluminum phosphide, Poisoning, G6PD deficiency, Protective effect, 6- aminonicotinamide (6-AN)
Subjects:QV pharmacology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:9105
Deposited By: Dr Ahmad Salimi
Deposited On:07 Jun 1396 05:28
Last Modified:24 Jan 1401 10:56

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