مکانیسم مولکولی متیل سولفونیل متان و آلیسین در مهار رشد سلولهای CD44± سرطان پستان

سرخانی, الهام and نجف زاده, نوروز and تاتا, نسرین and داستان, مریم and مأذنی, محمد and ارزنلو, محسن (1396) مکانیسم مولکولی متیل سولفونیل متان و آلیسین در مهار رشد سلولهای CD44± سرطان پستان. Journal of Functional Foods ــ 39 . pp. 50-57. شاپا 1756-4646

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Molecular mechanisms of methylsulfonylmethane and allicin in the inhibition of CD44± breast cancer cells growth

English Abstract

Breast carcinoma is a common cause of cancer death in women worldwide. CD44± cells were isolated from MCF7 cell line by magnetic-activated cell sorting (MACS) and treated with methylsulfonylmethane (MSM), allicin, and the combination of them. The cytotoxicity and cell cycle arrest were measured using MTT assay and flow cytometry. Moreover, mRNA levels of apoptosis regulators Bax, p53, and caspase-3 were measured using reverse transcriptase-polymerase chain reaction (RT-PCR). The combination treatment inhibited CD44� and CD44+ cells in the G2/M and S phases of the cell cycle, respectively.Importantly, Bax expression was significantly higher in the MSM/allicin-treated CD44+ cells than in the MSM- or allicin-treated cells (P < .05). The combination treatment enhanced more caspase-3 mRNA expression than allicin alone in both CD44± cells. Taken together, the combination treatment with MSM and allicin mediated cytotoxicity through modulating the expression of the key apoptotic factors

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :الهام سرخانی
نویسنده مسئول :نوروز نجف زاده
نویسنده :محمد مأذنی
نویسنده :محسن ارزنلو
Additional Information:Impact Factor (2016) 3.144 Indexed in: ISI, Scopus, Current Contents/Agriculture, Biology & Environmental Sciences, ScienceDirect, Scirus
کلیدواژه ها (انگلیسی):CD44; Breast carcinoma; Allicin; Methylsulfonylmethane; Apoptosis; Cell cycle arrest
Subjects:QV pharmacology > QV 744 Medicinal Chemistry
QV pharmacology
WD Nutrition Disease and metabolic diseases
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:9249
Deposited By: Dr Nowruz Najafzadeh
Deposited On:29 Jul 1396 11:11
Last Modified:30 Feb 1397 14:53

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