مطالعه بر روی پاسخ های ایمنی القا شده توسط DNAواکسن کد کننده آنتی ژنهای Mtb32C-HBHA از مایکوباکتریوم توبرکلوزیس

تیمورپور, رقیه and پیری دوگاهه, هادی and تیمورپور, امیر and ارزنلو, محسن and مشکات, زهرا (1396) مطالعه بر روی پاسخ های ایمنی القا شده توسط DNAواکسن کد کننده آنتی ژنهای Mtb32C-HBHA از مایکوباکتریوم توبرکلوزیس. Iranian Journal of Basic Medical Sciences ــ 20 (10). pp. 1119-1124. شاپا 2008-3866


Official URL: http://ijbms.mums.ac.ir/article_9445.html


A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis

English Abstract

Objective(s): Tuberculosis (TB) has still remained a global health issue. One third of the world's population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses against Mycobacterium tuberculosis. Materials and Methods: A DNA plasmid vaccine expressing Mtb32C-HBHA fusion protein was constructed and its ability in protein expression was examined by RT-PCR and Western blot methods. Female BALB/c mice were vaccinated with 100 μg of purified recombinant vector in an attempt to assess its immunogenicity and protective efficacy. Further, the cytokines, IFN-γ, IL-12, IL-4, IL-10, and TGF-β were assessed. Results: The levels of all the studied cytokines were significantly increased (P<0.05) compared with the control group. IFN-γ production in the group receiving DNA vaccine plus BCG was increased compared with those receiving only DNA vaccine or BCG (P<0.001). Conclusion: The immunogenicity of the new chimeric DNA vaccine was confirmed alone and in combination with BCG. Based on the results of the current study, the constructed DNA vaccine induced the expression of Mtb32C-HBHA fusion protein efficiently in vitro. Furthermore, high levels of the specific cytokines were induced in mice. By using this DNA vaccine as a booster after BCG, higher amounts of IFN-γ will be produced.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :رقیه تیمورپور
نویسنده :هادی پیری دوگاهه
نویسنده :محسن ارزنلو
نویسنده مسئول :زهرا مشکات
Additional Information:Impact Factor (2016) 1.424 Indexed in: ISI, Pubmed/PMC, Scopus, BIOSIS Previews , EMBASE, ISC, DOAJ, Google Scholar, Magiran
کلیدواژه ها (انگلیسی):BCG; DNA; Mycobacterium tuberculosis; PCR; Plasmid
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:9293
Deposited By: Dr Roghayeh Teimourpour
Deposited On:22 Aug 1396 09:23
Last Modified:06 May 1399 09:01

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