بررسی تنوع ژنتیکی گونه های اکینوکوکوس در سگ سانان اهلی و وحشی استان خراسان شمالی، شمال شرق ایران

عنوان انگليسی

Genetic variation of Echinococcus species in domestic and wild canines of North Khorasan Province, northeast Iran

خلاصه انگلیسی

Background: Canids are definitive hosts for Echinococcus species, causative agents of echinococcosis. This study aimed to determine genetic variation of Echinococcus species in domestic and wild canines of North Khorasan Province, northeast Iran. Methods: During 2013 and 2014, the carcasses of 106 canines were collected from the study area and examined for infectivity with Echinococcus species. DNA from Echinococcus samples were extracted using tissue DNA extraction kit. The cox1 and nad1 mitochondrial genes were amplified and sequenced. Alignments of the sequences, comparisons with registered sequences in GenBank, and phylogenetic analysis were performed. Intra-species and inter-species diversity were calculated. Results: Echinococcus granulosus were found in jackal (3.3%), dog (21.1%) and wolf (33.3%). Echinococcus multilocularis were found in jackal (13.1%) and fox (8.7%). All isolates of E. granulosus identified as G1 genotype. Based on the comparative analysis of Echinococcus sequences homology, intra-species genetic diversity for pcox1 and pnad1 within E. granulosus isolates were 0-0.7% and none, respectively; and these amounts within E. multilocularis isolates were none and 0-0.2%, respectively. Conclusion: Analysis of pcox1 and pnadl of Echinococcus isolates revealed circulation of both E. granulosus and E. multilocularis in the canids of the study area.

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