تجزیه و تحلیل اثرات سیتوتوکسیک نیکل بر روی لنفوسیت های خون انسان

زارعی, محمد هادی and حسینی شیرازی, سید فرشاد and اقوامی, مرجان and سلیمی, احمد and پوراحمد, جلال (1397) تجزیه و تحلیل اثرات سیتوتوکسیک نیکل بر روی لنفوسیت های خون انسان. Toxicology Mechanisms and Methods ــ 28 (2). pp. 79-86. شاپا 6516-1537

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Official URL: http://www.tandfonline.com/doi/full/10.1080/153765...


Analysis of cytotoxic effects of nickel on human blood lymphocytes

English Abstract

Nickel compounds possess many applications in different industrial processes. Human beings are exposed to nickel commonly through occupational exposure and food. Although a few studies so far have investigated the effects of nickel compounds on human lymphocytes, the complete mechanism of cytotoxicity of this metal on human lymphocytes is yet to be determined. The intention of this paper was to determine the cytotoxicity mechanism of water soluble NiCl2 toward human lymphocytes using the accelerated cytotoxicity mechanisms screening (ACMS) technique. Human lymphocytes were isolated from the blood of healthy subjects based on Ficoll-Paque PLUS standard method. For the assessment of cell viability, lymphocytes were incubated with 0.05–1 mM NiCl2 for 12 h. Determination of mechanistic parameters was performed 2, 4 and 6 h after treatment of cells with 1=2 EC5012h, EC5012h and 2EC5012h of NiCl2. Our results demonstrate that cytotoxicity of NiCl2 on human lymphocytes is associated with increased ROS formation, mitochondrial membrane potential collapse, glutathione depletion, lysosomal membrane damage, cellular proteolysis and activation of caspase-3 before cytotoxicity ensued.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محمد هادی زارعی
نویسنده :سید فرشاد حسینی شیرازی
نویسنده :مرجان اقوامی
نویسنده مسئول :احمد سلیمی
نویسنده مسئول :جلال پوراحمد
Additional Information:IF: 2.276 Indexed in: ISI, PubMed/Medline, Scopus, EMBASE
کلیدواژه ها (انگلیسی):Human lymphocytes; nickel; cellular proteolysis; cytotoxicity
Subjects:QV pharmacology > QV 600 Toxicology
QV pharmacology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:9468
Deposited By: Dr Ahmad Salimi
Deposited On:19 Sep 1396 07:25
Last Modified:24 Jan 1401 10:15

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