شناسایی مورفولوژیکی و مولکولی گونه های مزوسستوئیدس در میزبانان واسط(جوندگان) در ایران

عنوان انگليسی

Molecular characterization of Mesocestoides litteratus tetrathyridium isolated from meriones persicus and Cricetulus migratorius in Ardabil Province,Iran

خلاصه انگلیسی

Background: Mesocestoides spp. are Cyclophyllidean tapeworms with zoonotic importance. Molecular identifications have often been performed on adult stage in carnivore definitive hosts. However, in current study Mesocestoides larvae (tetrathyridium) isolated from abdominal Cavity of persion jird, Meriones persicus and from liver of grey hamster, Cricetulus migratorius in Ardabil Province, northwest Iran were subjected to molecular characterization. Methods: Genomic DNA of the isolates of Mesocestoides tetrathyridium were extracted and cytochrome-c oxidase subunit1 (cox1) mitochondrial gene was amplified. Sequencing of PCR products were performed and phylogenic analysis constructed, using MEGA 6.0 software. Results: Both isolates were identified as M. litteratus, showing 100% homology to each other and with sequences present in GenBank. Intra-species variation within isolates of M. litteratus amounted to 0-2.8%. The sequences were registered in GenBank with accession numbers KU182430.1 and KU182431.1. Conclusion: Based on morphological features M. lineatus has been frequently reported in Iran. This is the first molecular description of M. litteratus from M. persicus and C. migratorius. Phylogenetic analysis illustrated that M. litteratus isolates of current study had very low differences with the isolates of this species from other countries.

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