شناسایی مولکولی گونه های تریکیوریس جدا شده از رومبومیس اوپیموس در استان گلستان، ایران

عنوان انگليسی

Molecular identification of Trichuris species isolated from Rhombomys opimus in Golestan Province, Iran

خلاصه انگلیسی

Background: Species determination of Trichuris base on the morphology of adult females is difficult and molecular characterizations of this nematode can be helpful in this regard. In the current study, a female of Trichuris species isolated from great gerbil (Rhombomys opimus) in Golestan Province, Iran were characterized by nucleotide sequencing. Materials and methods: DNA of the adult Trichuris sp. was isolated by GeneAll Purification Kit and ITs1-5.8s-ITS2 region was amplified by NC5, NC2 primers. The PCR product was sent for nucleotide sequencing. Results: Alignments of the sequences of the current study, using Basic Local Alignment Search Tool, revealed existence of 99% homology between current isolate and Trichuris sp. H78 (Accession number: FN543202) isolate in GenBank from Rhombomys sp. in Kazakhstan. Conclusion: Due to genetic and morphological variability in genus Trichuris and influence of the host on this variation, it is necessary to use additional criteria, such as molecular analysis, for phylogenetic and taxonomic studies.

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