اثرات چاقی ناشی از رژیم غذایی بر روی پاسخ دهی تراشه به متاکولین، سطوح ویسفاتین تراشه، و تغییرات هیستولوژیک ریه در رتهای ویستار ماده حساس شده با اوآلبومین

کیهان منش, رعنا and علیپور, محمد رضا and ابراهیمی, هادی and اصلانی, محمدرضا (1397) اثرات چاقی ناشی از رژیم غذایی بر روی پاسخ دهی تراشه به متاکولین، سطوح ویسفاتین تراشه، و تغییرات هیستولوژیک ریه در رتهای ویستار ماده حساس شده با اوآلبومین. Inflammation ــ 41 (3). pp. 846-858. شاپا 0360-3997

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Official URL: https://link.springer.com/article/10.1007/s10753-0...


Effects of Diet-Induced Obesity on Tracheal Responsiveness to Methacholine, Tracheal Visfatin Level, and Lung Histological Changes in Ovalbumin-Sensitized Female Wistar Rats

English Abstract

Many studies have shown a close relationship between obesity and asthma severity. In the present study, the effects of diet-induced obesity were examined on airway responsiveness to methacholine in addition to visfatin level in female Wistar rats’ tracheae after sensitization with ovalbumin. The rats were divided into four groups: control with normal diet (ND), ovalbumin (OVA)-sensitized with normal diet (S + ND), high-fat diet (HFD), and OVA-sensitized with a high-fat diet (S + HFD). The animals were fed for 8 weeks with standard pelts or high-fat diet and then sensitized and challenged with OVA or saline for another 4 weeks. At the end of the study, the tracheae were isolated and assessed for airway responsiveness and visfatin protein levels. Diet-induced obesity groups developed increased weight and obesity indices (p < 0.001). After sensitization with OVA and diet-induced obesity, there were marked leftward shifts in methacholine concentration-response curves in S + HFD group compared to other groups. Also, maximum response was the highest (p < 0.05 to p < 0.001), EC50 was the lowest (p < 0.05 to p < 0.001), and visfatin protein level was the highest (p < 0.05 to p < 0.01) in S + HFD. According to results, diet-induced obesity caused airway hyperresponsiveness to methacholine and enhanced visfatin protein levels in the tracheae of ovalbumin-sensitized female rats. Our results suggested that, in obese ovalbumin-sensitized conditions in female rats, the local production of adipocytokines, such as visfatin, may be increased, resulting in the deterioration of inflammation in lungs. This finding shows a possible mechanism for the altered phenotype in obesity-ovalbumin sensitization conditions in female rats.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :رعنا کیهان منش
نویسنده :محمد رضا علیپور
نویسنده :هادی ابراهیمی
نویسنده مسئول :محمدرضا اصلانی
Additional Information:IF:2.88 Indexed in: ISI, PubMed/Medline, Scopus
کلیدواژه ها (انگلیسی):airway hyperresponsiveness, asthma ,obesity, visfatin, female Wistar rats
Subjects:QT physiology
WF Respiratory System
WK Endocrine System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:9733
Deposited By: Dr Mohammad Reza Aslani
Deposited On:15 Nov 1396 08:26
Last Modified:26 Jun 1402 13:08

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