تیمورپور, رقیه ، پیری دوگاهه, هادی ، ارزنلو, محسن ، قلوبی, آیدا ، سنکیان, مجتبی ، مشکات, زهرا (1396) بیان همزمان ژنهای hbha وMtb32C در سیستم پروکاریوت. jundishapur journal of microbiology ــ 11 (2). ص.ص.1-6. شاپا 2008-3645
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آدرس اینترنتی رسمی : http://jjmicrobiol.com/en/articles/14030.html
عنوان انگليسی
Co-Expression of hbha and mtb32C Genes from Mycobacterium tuberculosis H37Rv in a Prokaryotic System
خلاصه انگلیسی
Background: Heparin-binding hemagglutinin (HBHA) protein is a surface adhesin that mediates the attachment of Mycobacterium tuberculosis to host cells by its own unique, carboxyl-terminal region. The methylated HBHA has a specific motif with a lysine-, alanine-, and proline-rich domain. More recently, it has been shown that HBHA protein has potential activity in stimulating immune responses, and is a promising new candidate for diagnostic applications besides a protective antigen against tuberculosis. Objectives: The aim of this study was to isolate a mycobacterial latency gene (hbha), and subsequently produce its protein as a new antigen for the Interferon-Gamma Release Assay test (IGRAs). Methods: In the present work, hbha and mtb32C genes were isolated from the Mycobacterium tuberculosis H37Rv genome using the polymerase chain reaction (PCR) method. The PCR products and pet21+ vector were digested with specific restriction enzymes and then submitted to the ligation procedure. Escherichia coli BL21-CodonPlus (DE3) competent cells were transformed with the recombinant mtb32C-hbha -pet21+ vector. Expression of recombinant protein (Mtb32C-HBHA) was confirmed with Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and western blot methods. Results: Detection of a 500-bp gene and sequencing of recombinant pet-mtb32C-hbha vector, all confirmed the accuracy of the cloning procedure. A 36-KDa band of Mtb32C-HBHA protein was also detected by western blotting. Conclusions: In this study, expression of Mtb32C-HBHA protein was successfully done, in the prokaryotic system. Further studies are needed to evaluate the efficacy of recombinant Mtb32C-HBHA protein in diagnosis of latent tuberculosis.
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده اول : | رقیه تیمورپور |
نویسنده : | هادی پیری دوگاهه |
نویسنده : | محسن ارزنلو |
نویسنده مسئول : | زهرا مشکات |
ضریب تاثیر و نمایه مجلات: | Impact Factor (2016) 1.017 Indexed in: ISI, Scopus,Chemical Abstract , Embase, EBSCO, IndexCopernicous, Google Scholar, ISC, SID, Barakatkns , MagIran |
کلیدواژه ها (انگلیسی): | PCR; Interferon-Gamma Release Test; Heparin-Binding Hemagglutinin Protein; Mycobacterium tuberculosis |
موضوعات : | QW میکروب شناسی و ایمنی شناسی |
بخش های دانشگاهی : | دانشكده پزشكي > گروه علوم پایه > بخش میکروبیولوژی |
کد شناسایی : | 9937 |
ارائه شده توسط : | دکتر رقیه تیمورپور |
ارائه شده در تاریخ : | 01 اردبهشت 1397 12:58 |
آخرین تغییر : | 06 مرداد 1399 08:58 |
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