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عنوان انگليسی

NAAG reduces neutrophil infiltration and edema response in carrageenan-induced acute inflammation

خلاصه انگلیسی

Introduction: N-Acetylaspartylglutamate (NAAG) is widely distributed peptide transmitter in the mammalian nervous system, activates mGlu3 receptors at presynaptic sites which results to inhibit glutamate release. Evidence suggests that glutamate receptors are involved in inflammatory response carried through the COX-2 up regulation. In present study we aimed to investigate the effect of NAAG (10 mg kg-1) on carrageenan-induced acute inflammation. Methods: Acute inflammation was induced by subplantar injection of carrageenan 1% (w/v), in the right hind paw of the rats. The animals received an intraperitoneal injection of NAAG 20 min before induction of inflammation. The severity of inflammation was assessed 4 h later using maximum paw edema volume, Area Under Curve(AUC), microscopic changes of inflamed paw, measurement of oxidative stress markers; myeloperoxidase(MPO), lipid peroxidation(MDA level), SOD and GPx activity in paw tissue. Results and conclusion: NAAG reduced maximum inflammatory response and AUC by 52.56 and 58% (P\0.001), respectively, compare to control. Microscopic studies indicated significant reduction in neutrophil infiltration. MPO activity in NAAG treated group was inhibited by 80.19% (P\0.01) and MDA level was decreased by 88.13% (P\0.01) compare to controls, but there were no significant reduction in SOD and GPx activity. The results, for the first time, suggest that NAAG may be involved in inhibiting peripheral inflammation and it is possible that exerts this effect by modulating systemic glutamate levels.

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