مراکز الکترواکتیو آنزیم های آمین اکسیداز شیره افوربیا و جوانه عدس

هلی, حسین and امانی, مجتبی and موسوی موحدی, علی اکبر and جباری, علی and فلوریس, جیووانی and مورا, آنا (1387) مراکز الکترواکتیو آنزیم های آمین اکسیداز شیره افوربیا و جوانه عدس. Bioscience, Biotechnology and Biochemistry ــ 72 (1). pp. 29-36. شاپا 0916-8451

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Electroactive centers in Euphorbia latex and lentil seedling amine oxidases

English Abstract

The electrochemical behavior of redox centers in the active site of amine oxidases from lentil seedlings and Euphorbia characias latex was investigated using a mercury film electrode. Tyrosine-derived 6-hydroxydopa quinone (TPQ) and copper ions in the active site are redox centers of these amine oxidases. The enzymes undergo two reduction processes at negative potentials related to the reduction of the TPQ cofactor to the corresponding hydroquinones and the reduction of copper ions, (Cu(II) � Cu(I)). Copper depleted enzymes, prepared by reduction with dithionite followed by dialysis against cyanide, undergo only one reduction process. Nyquist diagrams, recorded at potentials corresponding to the reduction of cofactors as dc-offset, represent charge transfer impedance followed by a Warburg-type line at low frequencies, indicating the occurrence of a diffusion controlled process in the rate-limiting step of the reduction process.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :حسین هلی
نویسنده مسئول :علی اکبر موسوی موحدی
Additional Information:cited By (since 1996) 0 Indexed:1-Medline/pubmed 2-Scopuse
کلیدواژه ها (انگلیسی):Amine oxidase; Euphorbia latex; Lentil seedlings; Mercury film electrodes; Topaquinone, Charge transfer; Metal ions; Reduction, Enzyme activity, 6 hydroxydopa quinone; 6-hydroxydopa quinone; amine oxidase (copper containing); copper; DOPA; drug derivative; oxidoreductase; unclassified drug; vegetable protein, amino acid sequence; article; binding site; chemistry; electrochemistry; electrode; enzymology; Euphorbia; kinetics; lentil; metabolism; molecular genetics; oxidation reduction reaction; seedling, Amine Oxidase (Copper-Containing); Amino Acid Oxidoreductases; Amino Acid Sequence; Binding Sites; Copper; Dihydroxyphenylalanine; Electrochemistry; Electrodes; Euphorbia; Kinetics; Lens Plant; Molecular Sequence Data; Oxidation-Reduction; Plant Proteins; Seedling, Euphorbia; Euphorbia characias; Lens culinaris
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:1061
Deposited By: MS Soghra Golmaghani
Deposited On:13 Dec 1388 05:30
Last Modified:20 Jan 1393 13:03

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