تشکیل فیبریل در لیزوزیم در اثر اندرکنش با سدیم دودسیل سولفات درpH9.2

موسوی موحدی, علی اکبر and پیرزاده, پیمان and هاشم نیا, صدیقه and احمدیان, شهین and همتی نژاد, بهرام and امانی, مجتبی and صبوری, علی اکبر and احمد, فیضان and شمسی پور, مجتبی and حکیم الهی, غلامحسین and سای, فویوان and هادی علیجانوند, حمید and یوسفی, رضا (1386) تشکیل فیبریل در لیزوزیم در اثر اندرکنش با سدیم دودسیل سولفات درpH9.2. Colloids and Surfaces B: Biointerfaces ــ 60 (1). pp. 55-61. شاپا 0927-7765

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Fibril formation of lysozyme upon interaction with sodium dodecyl sulfate at pH 9.2

English Abstract

Fibril formation seems to be a general property of all proteins. Its occurrence in hen or human lysozyme depends on certain conditions, namely acidic pHs or the presence of some additives. This paper studies the interaction of lysozyme with sodium dodecyl sulfate (SDS) at pH 9.2, using UV-visible spectrophotometry, circular dichroism (CD) spectropolarimetry, electron microscopy (EM) and chemometry. Based on observations such as the strange increase in absorbance at 650 nm (pH 9.2) and the presence of intermediates, it is assumed that lysozyme fibrils have been formed at pH 9.2 in the presence of SDS as an anionic surfactant. Thioflavin T emission fluorescence and an EM image confirmed this assumption. β-cyclodextrin was then used as a turbidity inhibitor to establish its effect on the distribution of intermediates that participate in fibril formation. © 2007 Elsevier B.V. All rights reserved.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :علی اکبر موسوی موحدی
Additional Information:cited By (since 1996) 5
کلیدواژه ها (انگلیسی):Absorption; Anionic surfactants; Electron microscopy; pH effects; Spectrophotometers; Turbidity, Fibril formation; Sodium dodecyl sulfate, Enzymes, anionic surfactant; beta cyclodextrin; dodecyl sulfate sodium; lysozyme; thioflavine, acidity; article; chemometrics; circular dichroism; electron microscopy; fiber; fluorescence; image analysis; molecular interaction; pH; polarimetry; priority journal; spectrophotometry; turbidity; ultraviolet radiation, Animals; Chickens; Egg Proteins; Female; Hydrogen-Ion Concentration; Muramidase; Sodium Dodecyl Sulfate; Spectrophotometry, Ultraviolet; Surface-Active Agents
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:1063
Deposited By: MS Soghra Golmaghani
Deposited On:13 Dec 1388 06:15
Last Modified:20 Jan 1393 13:04

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