سطوح بیان ژنهای ER stress در بافت ریه رتهای نر و ماده چاق حساس شده با اوآلبومین

عنوان انگليسی

Expression level of endoplasmic reticulum stress genes in the lung tissue of obese male and female ovalbumin sensitized rats

خلاصه انگلیسی

Background: Human and animal studies have shown a close relationship between obesity and the severity of asthma. In this study, we investigated the expression of endoplasmic reticulum stress (ER stress) genes in the lung tissue of obese male and female ovalbumin sensitized rats. Methods: The rats were divided into eight groups (n=5 per group): female with the normal diet (F+ND), male with the normal diet (M+ND), female OVA-sensitized with the normal diet (F+SND), male OVA-sensitized with the normal diet (M+SND), female with high-fat diet (F+HFD), male with high-fat diet (M+HFD), female OVA-sensitized with high-fat diet (F+SHFD), and male OVA-sensitized with high-fat diet (M+SHFD). All rats were fed for 8 weeks with high-fat diet or standard pelts, and for another 4 weeks, they were sensitized with OVA or saline. At the end of the study, the tracheal responsiveness to methacholine, the ATF4, GRP78, XBP-1, and CHOP expression were determined by Real Time-PCR. Results: OVA-sensitization and diet-induced obesity caused the curve of methacholine concentration response to shifting to the left. In addition, results indicated that the EC50 (the effective concentration of methacholine generating 50% of peak response) in F+SHFD rats was statistically lower than M+SHFD group (p<0.05). Moreover, the results showed that diet-induced obesity increased the expression of ATF4, GRP78, XBP-1 and CHOP in the experimental model of asthma, markedly in F+SHFD group. Conclusion: These results suggest that ER stress may be involved in the pathogenesis of obesity associated with OVA-sensitized rat’s condition, especially in female animals.

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