سطوح سرمی ویسفاتین، سرتوین 1 و اینترلوکین 6 در بیماران با COPD در فاز Stable و exacerbation

عنوان انگليسی

Serum Levels of Visfatin, Sirtuin-1, and Interleukin-6 in Stable and Acute Exacerbation of Chronic Obstructive Pulmonary Disease

خلاصه انگلیسی

Background and Objective: Cardiac ischemic diseases are the leading cause of death in the world. Ischemia reperfusion injury (IRI) is a result of physical and biochemical disorders which occurs as a result of restoration of blood flow to the ischemic region. Stem cell therapy is a promising therapeutic target for improving myocardial injury. Human amniotic membrane mesenchymal cells (hAMCs) lead to the production and releasing of factors (condition medium) that are able to improve myocardial ischemic regions. But the exact mechanism of their therapeutic effects is unclear. Therefore, we investigated the effects of intra-myocardial injection of hAMCs-condition medium on myocardial interstitial fibrosis in IRI model in rats, Materials and Methods: Male Wistar rats (15 rats weighing 200-250g) were randomly divided into 3 groups: sham, IRI and IRI + condition medium. MI animals were subjected to 30 minutes left anterior descending coronary artery (LAD) ligation and 28 days of reperfusion. 150 μl of hAMCs-condition medium were injected into 3 different sites of the infarct border zone after 30 minutes of ischemia in treatment group. Left ventricle was used to measure cardiac fibrosis by Masson’s trichrome staining. Results: Interstitial fibrosis in IRI group was significantly higher than sham group (P < 0.05). Intra-myocardial injection of hAMCs-condition medium significantly decreased myocardial interstitial fibrosis (P < 0.05). Conclusion: The results of this study showed that hAMCs-condition medium has cardioprotective effects in IRI condition. Attenuation of myocardial interstitial fibrosis by hAMCs-condition medium plays significant role in this context.

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