غفرانی, مهدیه ، رضایی شیرمرد, لیلا ، امینی, محسن ، درکوش, فرید (1398) طراحی و فرمولاسیون نانوپارتیکلهای هپارین-کایتوزان حاوی اکترئوتاید: ارزیابی اثر تغییرات سطحی در خصوصیات فیزیکوشیمیایی و برداشت ماکروفاژها. Journal of Pharmaceutical Sciences ــ 108 (9). ص.ص.3036-3045. شاپا 0022-3549
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آدرس اینترنتی رسمی : https://www.jpharmsci.org/article/S0022-3549(19)30...
عنوان انگليسی
Development of Octreotide-Loaded Chitosan and Heparin Nanoparticles: Evaluation of Surface Modification Effect on Physicochemical Properties and Macrophage Uptake
خلاصه انگلیسی
Octreotide (OCT) is a therapeutic peptide which is administered for the treatment of acromegaly. The purpose of this study was to design a new polyethylene glycol (PEG)econjugated nanoparticle (PEG-NP) to overcome the short half-life and poor stability of OCT. The developed PEG-NPs were compared with non-PEGylated NPs with respect to their size, morphological characteristics, loading efficiency, release profile, and macrophage uptake. The OCT-loaded NPs and PEG-NPs were prepared by ionic complexion of chitosan (Cs) with either heparin (Hp) or PEGylated heparin (PEG-Hp). The chemical structure of PEG-Hp was confirmed by IR and proton nuclear magnetic resonance. Morphological analyses by scanning electron microscopy showed that NPs and PEG-NPs have a uniform shape. Dynamic laser scattering measurements indicated that hydrodynamic diameter of NPs and PEG-NPs were 222.5 ± 10.0 nm and 334.9 ± 6.7 nm, respectively. NPs and PEG-NPs had a positive zeta potential of about 32.5 ± 1.1 mv and 20.6 ± 2.4 mv, respectively. Entrapment efficiency was 61.4 ± 1.0% and 55.7 ± 2.4% for NPs and PEG-NPs, respectively. Compared with the NPs, the PEG-NPs exhibited a slower release profile. Subsequently, fluorescein isothiocyanateelabeled chitosanCs was synthesized and used to evaluate the stealth characteristic of PEG-NPs. In vitro macrophage uptake of fluorescently labeled NPs was measured by flow cytometry
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده اول : | مهدیه غفرانی |
نویسنده : | لیلا رضایی شیرمرد |
نویسنده : | محسن امینی |
نویسنده مسئول : | فرید درکوش |
ضریب تاثیر و نمایه مجلات: | IF: 3.197 Indexed in:ISI, PubMed/Medline, Scopus, Embase |
کلیدواژه ها (انگلیسی): | chitosan, heparin, peptide delivery, polyethylene glycol, drug delivery system(s) |
موضوعات : | QV فارماکولوژی > QV 704 فارماسیوتیکس QV فارماکولوژی |
بخش های دانشگاهی : | دانشکده داروسازی > بخش فارماسیوتیکس |
کد شناسایی : | 12640 |
ارائه شده توسط : | دکتر لیلا رضائی |
ارائه شده در تاریخ : | 06 بهمن 1398 13:40 |
آخرین تغییر : | 06 بهمن 1398 13:40 |
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