اثر استرادیول بر سمیت ناشی از تری بوتیل تین در جزایر لانگرهانس موش صحرایی

عنوان انگليسی

The effect of estradiol against tributyltin toxicity in rat pancreatic islets

خلاصه انگلیسی

Introduction: Tributyltin is known as an endocrine disruptor and has been reported to interfere with estrogen receptors exerting its toxic effects through disrupting related endocrine pathways. It is known as an “obesogen” and was reported to disrupt glucose homeostasis leading to diabetes. This study was designed to assess the effects of tributyltin and estradiol on rat pancreatic islets. Methods: Pancreatic islets of male rat were isolated, grouped (10 islets in each group) and cultured in RPMI 1640 for 24 hours at 37° C. After calculating EC50 of tributyltin and estradiol by using MTT assay, islets were treated with estradiol and tributyltin for 24 hours. Then viability and level of reactive oxygen species (ROS), Insulin secretion and apoptosis were measured. Results: Tributyltin decreased cellular viability of islets along with an increase in the ROS formation, while estradiol increased viability and decreased ROS when added to both control group and tributyltin treated group. Tributyltin also increased insulin secretion and induced apoptosis, while estradiol caused a decrease in insulin secretion and apoptosis. Conclusion: Our results indicate that estradiol can protect islets of the pancreas by inhibiting apoptosis and decreasing ROS as well as insulin secretion. It can be concluded that function of estrogen receptors in the pancreas particularly β-cells might be an important target for pharmacological and toxicological modifications in order to discover new aspects of pathophysiology and therapeutic strategies for diabetes. Keywords: Islets of Langerhans, Estrogen receptors, Estradiol, Tributyltin

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