title

تشخیص -652 6N INS/del در ژن کاسپاز 8 در بیماران مبتلا به سرطان سینه با استفاده از روش Bi-PASA

هاشمی, محمد and اسکندری نسب, ابراهیم and فضایلی, علی اکبر and رضایی, حمزه and مشهدی, محمد علی and اربابی, فرشید and طاهری, محسن (1391) تشخیص -652 6N INS/del در ژن کاسپاز 8 در بیماران مبتلا به سرطان سینه با استفاده از روش Bi-PASA. Gene ــ 505 (1). pp. 176-179. شاپا 0378-1119

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Official URL: https://doi.org/10.1016/j.gene.2012.05.043


Title

Bi-directional PCR allele-specific amplification (bi-PASA) for detection of caspase-8 − 652 6N ins/del promoter polymorphism (rs3834129) in breast cancer

English Abstract

Caspase-8 (CASP8) plays a critical role in regulating apoptosis, and its functional polymorphisms may modify cancer risk. We investigated the possible association between CASP8 −652 6N ins/del (rs3834129) and the risk of breast cancer in a sample of Iranian population. This case–control study was done on 236 breast cancer patients and 203 cancer free healthy female. We designed a rapid and simple bi-directional PCR allele-specific amplification (bi-PASA) for detection of CASP8 −652 6N ins/del polymorphism. The results showed that the CASP8 −652 6N del/dl genotype was inversely associated with breast cancer risk (OR=0.33, 95% CI=0.17– 0.65, p=0.001). The frequencies of the del allele in cases and controls were 29.1% and 38.6%, respectively. An inverse association between CASP8 6N del variant and the risk of breast cancer (OR=0.66, 95% CI=0.66– 0.87, p=0.002) was found. In conclusion, the result suggests that the CASP8 −652 6N del polymorphism plays a protective role in susceptibility to breast cancer in our population. Further studies in other populations with larger samples are needed to confirm these findings.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :محمد هاشمی
نویسنده :ابراهیم اسکندری نسب
نویسنده :علی اکبر فضایلی
نویسنده :حمزه رضایی
نویسنده :محمد علی مشهدی
نویسنده :فرشید اربابی
نویسنده :محسن طاهری
Additional Information:IF: 2.196 Indexed in: ISI, PubMed/Medline, Scopus, Embase
کلیدواژه ها (انگلیسی):Caspase-8 , Breast cancer, Polymorphism
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
ID Code:13927
Deposited By: Dr. Aliakbar Fazaeli
Deposited On:13 Nov 1399 10:03
Last Modified:13 Nov 1399 11:25

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