اثر اینکوباسیون بافت با ایزوپرنالین بر پاسخ های تراشه سالم و فاقد اپی تلیوم نسبت به متاکولین

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عنوان انگليسی

THE EFFECT OF TISSUE INCUBATION WITH ISOPRENALINE ON RESPONSES OF INTACT AND DENUDED EPITHELIUM TRACHEA TO METHACHOLINE

خلاصه انگلیسی

There are reports regarding desensitization of B2 receptors in asthmatic patients. Epithelial shedding of airway is a pathological feature of asthma. In the present study, the responses of tracheal chains of intact and denuded epithelium (groups 1 and 2, n=10 for each group) in the incubation (with 10 µM isoprenaline, during 1h resting period) and non-incubation design to cumulative concentration of methacholine were measured, and EC50 was obtained. The same protocol was performed in groups 3 and 4 (n=5 for each group) which were similar to groups 1 and 2 except there was an additional 30 min rest in incubated design before pharmacological measurements. The response of denuded epithelium trachea to methacholine (3.93±0.62) was significantly higher than that of intact epithelium (9.36±2.60, P<0.05) in non-incubated condition. Incubation to isoprenaline caused significant reduction in tracheal response to methacholine in both the denuded (8.75±1.33) and intact epithelium (18.20±4.03, P<0.005 for both cases). The response of denuded epithelium trachea to methacholine in incubated condition was also significantly higher than that of intact epithelium (P<0.05). Furthermore, the reduction in tracheal responsiveness to methacholine due to incubation in epithelium denuded group was no significantly greater than that of intact epithelium group. There was no any significant difference between groups 3 and 4 with those of groups 1 and 2 in both incubated and non-incubated conditions. The maximum contractility response to methchoine was not significantly different between tracheal chains with denuded and intact epithelium and did not change to due to incubation with isoprenaline. The results of this study indicate reduction of tracheal response to methachline due to incubation of tissues with isoprenaline, which was relatively more pronounced in epithelium denuded trachea.

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• اثر اینکوباسیون بافت با ایزوپرنالین بر پاسخ های تراشه سالم و فاقد اپی تلیوم نسبت به متاکولین. (deposited 20 اردبهشت 1400 07:15) [Currently Displayed]

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