شواهد افزایش سلولهای T تنظیم کننده قبل از متاستاز در بیماران مبتلا به سرطان ریه Non small cell lung cancer

عرفانی, نصراله and قیومی, محمدعلی and مفخمی, شایسته and امانی, داور and حق شناس, محمدرضا and قادری, عباس (1389) شواهد افزایش سلولهای T تنظیم کننده قبل از متاستاز در بیماران مبتلا به سرطان ریه Non small cell lung cancer. در: دهمین کنگره بین المللی ایمنولوژی و آلرژی ایران, 28-30 اردیبهشت 1389, ایران - تهران دانشگاه علوم پزشکی شهید بهشتی.

Text - Published Version


Evidences of T reg cells increase before metastasis in patients with Non-Small cell lung cancer

English Abstract

Background and Objectives:Recent studies have shown that the function of regulatory T Cells (T reg) play a pivotal role in suppression of the effective immune responses against cancer . The purpose of the study was to evaluate the prevalence of CD4+CD25+FOXP3 Treg cells on both metastatic and none -metastatic stages of non small cell lung cancer(NSCLC) patients and Healthy controls. Methods:Twenty one new cases with NSCLC who received no prior treatment as well as sixteen sex-age matched healthy donors were recruited. Mono nuclear cells were isolated and intracellular staining for FOXP3 and surface staining of CD4 and CD25 molecules followed by flowcytometric analysis was used to evaluate the prevalence of the targeted cells. Results: Compared with healthy donors¸ NSCLC patients had an increased percentage of Regulatory T cells than controls (7.91 ± 4.13 vs 3.85 ± 1.76 ¸ p<0.05). The proportion of Treg cells in the patients was directly proportional to stage increase (stage II = 5.16 ± 2.4¸ stage III= 7.94 ± 4.3¸stage IV=11.96 ± 2.2 ¸ p<0.05) and was also significantly higher in metastatic than non metastatic stages (11.96 ± 2.22 vs 6.83 ± 3.86¸ p<0.05). Total T cells from the patients had significantly higher CTLA4 expression both in intracellular compartments and on the surface. (Intracellular: 19.2 ±17 vs 7.32 ± 12.2, Surface: 0.71± 0.56 vs 0.46 ± 0.19¸ p<0.05). Conclusion:our data not only verify the previous studies of T reg cells as increased in the NSCLC patients but also provided new insight in the relation of Metastasis and T reg cell increase. The results indicated that a significant increase in T reg cells precede, and accordingly may accelerate,metastasis;a finding which suggest the use of T reg targeted immunotherapy with late stages of cancer before metastasis.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :نصراله عرفانی
Uncontrolled Keywords:سلولهای T تنظیمی ،سرطان سلولهاي غير كوچك ريه، پاسخ ایمنی
کلیدواژه ها (انگلیسی):T regulatory cells, non small cell lung cancer, Immune response
Subjects:QZ Pathology
QW Microbiology and Immunology
WF Respiratory System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Immunology
ID Code:1668
Deposited By: Dr Davar Amani
Deposited On:21 Jul 1389 00:06
Last Modified:07 Jul 1393 05:08

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