اصلانی, محمدرضا ، جعفری, زهرا ، رهبرقاضی, رضا ، رضایی, جعفر ، دلخوش, عارف ، احمدی, مهدی (1401) تاثیرات کروسین بر نسبت T-bet/GATA-3 و سطوح بیان miR-146a و miR-106a در بافت ریه موش های حساس شده با اوآلبومین. Iranian Journal of Basic Medical Sciences ــ 25 (10). ص.ص.1267-1274. شاپا 2008-3866
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آدرس اینترنتی رسمی : https://ijbms.mums.ac.ir/article_21060.html
عنوان انگليسی
Effects of crocin on T-bet/GATA-3 ratio, and miR-146a and miR- 106a expression levels in lung tissue of ovalbumin-sensitized mice
خلاصه انگلیسی
Objective(s): Although various studies have revealed the beneficial effects of crocin (derived from saffron), such as anti-inflammatory, anti-cancer, antioxidant, and immune modulator, however, its exact mechanism is unknown. The present study aimed to investigate the effect of crocin on the expression ratio of T-bet/GATA-3 as an indicator of altered immune responses in the lung tissue of ovalbumin (OVA)-sensitized mice. In addition, the effect of crocin on the expression level of miR-146a and miR-106a in the lung tissue OVA-sensitized mice was investigated.Materials and Methods: Mice were randomly divided into five groups (n=6): Control; OVA, OVA + Crocin 25, OVA + Cro 50, and OVA + Cro100 groups. Crocin was administrated intraperitoneally at doses of 25, 50, and 100 mg/kg for five consecutive days. One day after asthma induction, animals were euthanized, and lungs were sampled for pathological and gene expression analysis.Results: OVA-sensitization led to increased inflammation and histopathological changes in the lung tissue of mice. In addition, GATA-3 expression increased (P<0.001) and T-bet expression decreased (P<0.001) in OVA-sensitized groups. The T-bet/GATA3 ratio was also reduced markedly in asthma groups (P<0.001). Furthermore, increased expression of miR-146a and miR-106a levels was evident in the lung tissue of OVA-sensitized mice (P<0.001 for both). Intervention with high concentrations of crocin (50 and 100 mg/kg) significantly reduced airway inflammation, GATA-3 expression, miR-146a expression, and miR-106a expression and corrected the T-bet/GATA-3 ratio (P<0.05 to P<0.001).Conclusion: Treatment with crocin led to a decrease in the severity of lung inflammation in OVA -sensitized mice, which is probably through the reduction of the T-bet/GATA-3 ratio, and mir-146a and mir-106a expression level.
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده اول : | محمدرضا اصلانی |
نویسنده : | زهرا جعفری |
نویسنده : | رضا رهبرقاضی |
نویسنده : | جعفر رضایی |
نویسنده : | عارف دلخوش |
نویسنده مسئول : | مهدی احمدی |
ضریب تاثیر و نمایه مجلات: | IF: 2.532 Indexed in: ISI, Scopus, PubMed/PMC, Embase |
کلیدواژه ها (انگلیسی): | GATA-3 , microRNA-106a, microRNA-146a, T-box transcription factor |
موضوعات : | QT فیزیولوژی |
بخش های دانشگاهی : | دانشكده پزشكي > گروه علوم پایه > بخش فیزیولوژی |
کد شناسایی : | 16806 |
ارائه شده توسط : | دکتر محمد رضا اصلانی |
ارائه شده در تاریخ : | 10 اسفند 1401 09:28 |
آخرین تغییر : | 10 اسفند 1401 09:28 |
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