ارزیابی چرخه سلولی سلولهای سرطان انسانی به وسیله فلوسایتومتری به دنبال تاثیر دی متیل سولفون

Title

Flow cytometric analysis of the effect of dimethylsulfone on cell cycle arrest in human cancer cell lines

English Abstract

Introduction: Dimethylsulfone (MSM) is a dietary supplement to support healthy body. It has been suggested that MSM has a chemopreventive mechanism that affects the interaction of tumor cells with the host immune response. Considering preventive effects of this substance on tumor onset and non-toxic to healthy body we investigated in vitro effects of dimethylsulfone on cell cycle distribution in cancer cell lines. Materials and methods: Cells were seeded in 25 cm2 flasks at a density of 1×106 cells/flask. Cells were treated with MSM for 24 h at a range of concentration. After treatment, the DNA content and cellcycle distribution were determined by flow cytometry. Briefly, cells were trypsinized and harvested, then cells fixed with 70% ice ethanol. Fixed cells were centrifuged and washed with cold PBS, and then stained with DAPI 10 μg/ml. The stained cells were then transferred to flow tubes by passing through a nylon mesh with a pore size of 30 μm. Results: The percentage of cells in G1, S and G2/M phase was calculated using Partec FloMax software. We observed a dosedependent effect of MSM on the cell cycle. After 24 h of MSM treatment at different concentrations, cells in the G2/M population increased compared to controls. The effect of MSM on cancer cells appears to be dose-dependent. The higher the dosage, the greater the G2/M population increase. Conclusion: The results showed that MSM is able to induce a G2 M cell cycle arrest in gastrointestinal cancer cell lines

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