بررسی مولکولی آنزیم های β – لاکتاماز طیف گسترده(PER-1 (ESBL ، GES-1و CTX-M در سویه های پسودوموناس آئروژینوزا جداشده ازنمونه های بالینی

Title

Molecular Detection of Extended Spectrum β-Lactamase (ESBL) Genes PER-1, GES-1 &CTX-M in Clinical Isolate of Pseudomonas aeruginosa

English Abstract

Background & Objectives: Hospital-acquired infections caused by Pseudomonas aeruginosa are so intense and have a high mortality rate .Since that resistance to extended spectrum Cephalosporins in P. aeruginosa due to achieving the ESBLs genes being increasingly reported worldwide , this study aimed to consider molecular prevalence of ESBL genes blaPER-1 ,blaGES-1 &blaCTX-M in clinical isolate of P. aeruginosa strains. Methods: Genome of 110 p. aeruginosa strains isolated from a variety of clinical samples such as sputum ,blood and urine were extracted then the presence of ESBLs genes blaPER-1 ,blaGES-1 & blaCTX-M were analyzed by polymerase chain reaction (PCR) with choosing three pairs primers. Results: According to PCR result among 110 considered P. aeruginosa strains ,bla PER-1 was detected in 33.6%(37/110) ,blaGES-1 was detected in 39% (43/110) and bla CTX-M was detected in 65.4% (72/110) and among all strains 15.4%( 17 /110) appeared to produce each three genes . Conclusion: In the present study it was confirmed that a high spreading of ESBL genes exist in clinical P. aeruginosa strains , in addition bla CTX-M ESBLs gene is the most prevalent gene ,therefore performance of antibiogram test is necessary before using antibiotics

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