بررسی مولکولی آنزیم های β – لاکتاماز طیف گسترده(PER-1 (ESBL ، GES-1و CTX-M در سویه های پسودوموناس آئروژینوزا جداشده ازنمونه های بالینی

ستاریان, فرشته and ایمانی فولادی, عباسعلی and موسوی, سید فضل الله and صدیقیان راد, حمید (1391) بررسی مولکولی آنزیم های β – لاکتاماز طیف گسترده(PER-1 (ESBL ، GES-1و CTX-M در سویه های پسودوموناس آئروژینوزا جداشده ازنمونه های بالینی. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Molecular Detection of Extended Spectrum β-Lactamase (ESBL) Genes PER-1, GES-1 &CTX-M in Clinical Isolate of Pseudomonas aeruginosa

English Abstract

Background & Objectives: Hospital-acquired infections caused by Pseudomonas aeruginosa are so intense and have a high mortality rate .Since that resistance to extended spectrum Cephalosporins in P. aeruginosa due to achieving the ESBLs genes being increasingly reported worldwide , this study aimed to consider molecular prevalence of ESBL genes blaPER-1 ,blaGES-1 &blaCTX-M in clinical isolate of P. aeruginosa strains. Methods: Genome of 110 p. aeruginosa strains isolated from a variety of clinical samples such as sputum ,blood and urine were extracted then the presence of ESBLs genes blaPER-1 ,blaGES-1 & blaCTX-M were analyzed by polymerase chain reaction (PCR) with choosing three pairs primers. Results: According to PCR result among 110 considered P. aeruginosa strains ,bla PER-1 was detected in 33.6%(37/110) ,blaGES-1 was detected in 39% (43/110) and bla CTX-M was detected in 65.4% (72/110) and among all strains 15.4%( 17 /110) appeared to produce each three genes . Conclusion: In the present study it was confirmed that a high spreading of ESBL genes exist in clinical P. aeruginosa strains , in addition bla CTX-M ESBLs gene is the most prevalent gene ,therefore performance of antibiogram test is necessary before using antibiotics

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :فرشته ستاریان
کلیدواژه ها (انگلیسی):Pseudomonas aeruginosa ; ESBL ; PCR
Subjects:QW Microbiology and Immunology
WX Hospital and other health Facilities
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:4920
Deposited By: MS Soghra Golmaghani
Deposited On:26 Aug 1392 13:17
Last Modified:26 Aug 1392 13:17

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