شناسایی بوردتلا پاراپرتوسیس از نمونه های جدا شده از موارد بالینی بر اساس کشت و Real-Time PCR

عنوان انگليسی

Detection of Bordetella parapertussis in Clinical Samples Based on Real-Time PCR and Culture

خلاصه انگلیسی

Background & Objectives: Parapertussis is a bacterial illness that is similar to pertussis (whooping cough) but is typically milder than pertussis. Parapertussis is caused by the bacterium Bordetella parapertussis. Compared to pertussis, the occurrence of parapertussis is infrequent. However, because of lack of data about this illness, the aim of this research was phenotypic and molecular detection of B. parapertussis strains in clinical samples isolated from patients in Iran. Methods: Totally 1269 respiratory tract swabs were used for both culture and real-time PCR assay based on IS1001 target to rapid detection of B. parapertussis in the samples. Results: Among all specimens collected, 1 specimen was culture positive and 8 specimens were diagnosed as infected by B. parapertussis using IS1001 primers. One isolate that was positive with both culture and real-time PCR, was from a 6 years old patient who didn’t use any antibiotic before sampling. All PCR positive specimens were isolated from children 7 years of age or younger. Our data showed that 4 PCR positive specimens including culture positive one were from vaccinated children. Conclusion: Although B. parapertussis causes less sever disease compared to pertussis, this illness would be more sever among infants less than six months of age than older persons. However, as it's expected, the rate of infected persons by this organism was very low in this research. As whooping cough vaccines composed only of B. pertussis antigens, it provides little if any protection against B. parapertussis. Four infected patients were vaccinated in this study.

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