نیاپور, نازیلا and محمدی قلعه بین, بهنام and گل محمدی, محمد قاسم and امانی, محمد and صالحی, حسین and نیاپور, علی (1393) اثر بهینه سازی زمان پره دژنراسیون بر تعداد و خلوص سلول های شوآن حاصل از عصب محیطی سگ. Iranian Journal of Basic Medical Sciences ــ 18 (3). pp. 307-311. شاپا 2008-3866
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Official URL: http://ijbms.mums.ac.ir/article_4136_595.html
Title
Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures
English Abstract
Objective(s): Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve. Materials and Methods: Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The total cell yield and the purity of SCs were evaluated in each group on the first and seventh day after plating. Results: The maximum and minimum numbers of cells were counted in 15 day-long predegene-ration and control groups which underwent no predegeneration. The 10 day-long in vitro predegeneration group with 80±0.5% SCs enrichment had the best purity after plating day and could maintain its purity with elapsing on cultures. Conclusion: 10 day-long predegeneration results in the higher cell number and the better and prolonged purity of SCs in culture.
Item Type: | Article |
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زبان سند : | انگلیسی |
نویسنده اول : | نازیلا نیاپور |
نویسنده : | بهنام محمدی قلعه بین |
نویسنده : | محمد قاسم گل محمدی |
نویسنده : | محمد امانی |
نویسنده : | حسین صالحی |
نویسنده مسئول : | علی نیاپور |
Additional Information: | Impact Factor: (2014)1.228 Indexing in : ISI , Scopus , PubMed/PMC , EMBASE |
کلیدواژه ها (انگلیسی): | Dog; Sural nerve; Wallerian Degeneration; Duration; Schwann cell |
Subjects: | QS Human Anatomy WL Nervous system |
Divisions: | Faculty of Medicine > Department of Basic Sciences > Department of Anatomy Faculty of Medicine > Department of Basic Sciences > Department of physiology |
ID Code: | 6623 |
Deposited By: | Dr Ali Niapour |
Deposited On: | 06 Feb 1394 06:56 |
Last Modified: | 31 Jan 1400 09:47 |
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