title

اثر بهینه سازی زمان پره دژنراسیون بر تعداد و خلوص سلول های شوآن حاصل از عصب محیطی سگ

نیاپور, نازیلا and محمدی قلعه بین, بهنام and گل محمدی, محمد قاسم and امانی, محمد and صالحی, حسین and نیاپور, علی (1393) اثر بهینه سازی زمان پره دژنراسیون بر تعداد و خلوص سلول های شوآن حاصل از عصب محیطی سگ. Iranian Journal of Basic Medical Sciences ــ 18 (3). pp. 307-311. شاپا 2008-3866

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Title

Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures

English Abstract

Objective(s): Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve. Materials and Methods: Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The total cell yield and the purity of SCs were evaluated in each group on the first and seventh day after plating. Results: The maximum and minimum numbers of cells were counted in 15 day-long predegene-ration and control groups which underwent no predegeneration. The 10 day-long in vitro predegeneration group with 80±0.5% SCs enrichment had the best purity after plating day and could maintain its purity with elapsing on cultures. Conclusion: 10 day-long predegeneration results in the higher cell number and the better and prolonged purity of SCs in culture.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :نازیلا نیاپور
نویسنده مسئول :علی نیاپور
Additional Information:Impact Factor: (2014)1.228 Indexing in : Science Citation Index Expanded , BIOSIS Previews , PubMed Central , Scopus , EMBASE
کلیدواژه ها (انگلیسی):Dog; Sural nerve; Wallerian Degeneration; Duration; Schwann cell
Subjects:QS Human Anatomy
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:6623
Deposited By: Dr Ali Niapour
Deposited On:06 Feb 1394 06:56
Last Modified:05 Aug 1394 08:54

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