ارزیابی برون تنی و درون تنی نانوذرات پلیمری زیست تخریب پذیر

عنوان انگليسی

In vitro and in vivo evaluation of biodegradable PCL nanoparticles

خلاصه انگلیسی

Poly (caprolactone fumarate) (PCLF) copolymer has been synthesized by polycondensation of biodegradable, biocompatible and FDA approved poly (caprolactone) and fumaryl chloride and has been used for tissue engineering. In this study, ligand-less PCLF nanoparticles (NPs) were evaluated as a drug delivery system (DDS) with a potential of improved lymphoma tissue uptake regarding PCLF hydrophobicity. Doxorubicin HCl (Dox) release profiles showed a burst release of 27% during initial 6 hours following by a desired controlled release of 85% in 4 days in pH 5.8 (tumor pH). Empty PCLF NPs did not cause a considerable cytotoxicity on T47D, HT29 and 3T3NIH model cell lines, indicating the polymer safety. Cytotoxicities of Dox loaded PCLF NPs were almost equal to those of Dox alone revealing the NPs ability for tumor treatment. Fluorescent microscopy images proved an efficient uptake of PCLF NPs by T47D model cells. In vivo images showed two days accumulation of PCLF NPs in liver and spleen and specially in lymph node rich regions of mouse body after 24 hours of injection. Such characteristics made PCLF NPs DDS a promising candidate for treatment of lymphoma or other lymphatic disorders.

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