آزلائيك اسيداز طریق کاهش رشد فیبروبلاست می تواند به رژنراسیون اعصاب محیطی کمک کند

عنوان انگليسی

Azelaic acid could promote peripheral nerve regeneration through reducing fibroblast growth

خلاصه انگلیسی

Background and Aim: Traumatic injury to the peripheral nerve results in neural dysfunctions and neuropathy. A series of cellular and molecular events takes place before regeneration of injured peripheral nerves. During Wallerian degeneration, Schwann cellderived TNFα and IL-1α induce resident fibroblasts to further produce and liberate cytokines such as IL-6 and GM-CSF following 2 to 5 hours after the injury. Meanwhile, fibroblasts proliferate and accumulate at the injury site and may participate in neuroma tissue formation. Azelaic acid (AzA) which is being used for the treatment of inflammatory skin diseases like acne and rosacea has inhibitory effects on pro-inflammatory cytokines such as IL-1, IL-6 and TNF-α release. Therefore, the aim of this study was to evaluate the possible effects of the AzA on rat peripheral nerve fibroblasts viability. Methods: Rat peripheral nerve fibroblasts were seeded at a density of 1000 cells per well in 96 well plates. After 24 hours, cells were incubated with different concentrations of the AzA (from 0.1μM to 10 mM) for 48 hours. Cellular viability was determined using MTT assay. Results: Our findings demonstrated that after 48 hours of treatment with 2.5 and 5mM of AzA, significant reduction of cellular viability were observed. However, concentrations lower than 2.5mM showed minimal impacts on reducing rat peripheral nerve fibroblasts growth. The 5th Symposium of World Federation of 422 Neurosurgical Societies Conclusion: AzA had a cytotoxic effect in rat peripheral nerve fibroblasts. Our data provides an in vitro evidence of azelaic acid in prevention of neuroma tissue formation.

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