مهار رقابتی آمین اکسیداز حاوی مس توسط هیدروکلراید ویتامین B

طلایی, سونا and اسدی, اسداله and امانی, مجتبی (1392) مهار رقابتی آمین اکسیداز حاوی مس توسط هیدروکلراید ویتامین B. Molecular Biology Research Communications ــ 2 (1-2). pp. 29-38. شاپا 2322-181X

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Competitive inhibition of copper amine oxidases by vitamin B hydrochloride in chickpea

English Abstract

Copper amine oxidases (CAOs) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. The enzymes are homodimers containing both topaquinone and a Cu(II) ions as cofactors at the active site of each subunit. After extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, Km and Vmax of the enzyme were determined to be 3.3 mM and 0.95 mmol/min/mg, respectively, using a Lineweaver-Burk plot. In this study, the interaction of chickpea diamino oxidase with vitamin B hydrochloride was studied. Vitamin B hydrochloride (Thiamin) by Ki=8 mM acted as competitive inhibitor of the enzyme.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :اسداله اسدی
نویسنده :مجتبی امانی
Additional Information:Indexed in: DOAJ, Index Copernicus, ISC, SID, HINARI, Magiran, Animal Science Database (CABI), Academic Journals Database, Google Scholar
کلیدواژه ها (انگلیسی):words: Chickpea, Copper-containing amine oxidases, Vitamin B hydrochloride,Competitive inhibitor.
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:8033
Deposited By: Dr Mojtaba Amani
Deposited On:23 Aug 1395 05:37
Last Modified:23 Aug 1395 05:37

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