طلایی, سونا and اسدی, اسداله and امانی, مجتبی (1392) مهار رقابتی آمین اکسیداز حاوی مس توسط هیدروکلراید ویتامین B. Molecular Biology Research Communications ــ 2 (1-2). pp. 29-38. شاپا 2322-181X
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Title
Competitive inhibition of copper amine oxidases by vitamin B hydrochloride in chickpea
English Abstract
Copper amine oxidases (CAOs) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. The enzymes are homodimers containing both topaquinone and a Cu(II) ions as cofactors at the active site of each subunit. After extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, Km and Vmax of the enzyme were determined to be 3.3 mM and 0.95 mmol/min/mg, respectively, using a Lineweaver-Burk plot. In this study, the interaction of chickpea diamino oxidase with vitamin B hydrochloride was studied. Vitamin B hydrochloride (Thiamin) by Ki=8 mM acted as competitive inhibitor of the enzyme.
| Item Type: | Article |
|---|---|
| زبان سند : | انگلیسی |
| نویسنده مسئول : | اسداله اسدی |
| نویسنده : | مجتبی امانی |
| Additional Information: | Indexed in: DOAJ, Index Copernicus, ISC, SID, HINARI, Magiran, Animal Science Database (CABI), Academic Journals Database, Google Scholar |
| کلیدواژه ها (انگلیسی): | words: Chickpea, Copper-containing amine oxidases, Vitamin B hydrochloride,Competitive inhibitor. |
| Subjects: | QU Biochemistry |
| Divisions: | Faculty of Medicine > Department of Basic Sciences > Department of Biophysics |
| ID Code: | 8033 |
| Deposited By: | Dr Mojtaba Amani |
| Deposited On: | 23 Aug 1395 05:37 |
| Last Modified: | 23 Aug 1395 05:37 |
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