تجمع سلولهای بنیادی عصبی درونزاد در سری ترین قسمت بطن های طرفی مغز موش بالغ

عنوان انگليسی

The Majority of Endogenous Neural stem cells reside in the most rostral part of lateral ventricle in the adult mouse brain

خلاصه انگلیسی

Background: It is now clear that adult mammalian brain contains a population of neural precursor cells (neural stem and progenitor cells) that maintain homeostasis and can also replace lost cortical and subcortical neurons following injury. Objective: Given the paucity of information regarding the distribution of neural precursor cells in the adult mammalian brain we employed three distinct assays, the neurosphere assay (NSA), neural colony forming cell assay (N-CFCA) and label retaining cell (LRC) approach, to compile a detailed atlas of the frequency and distribution of these cells along the adult mouse brain ventricular neuraxis. Materials and Methods: Brains were harvested and serially Vibratome and Cryo sectioned (400 and 14 μm/section respectively) starting at the level of the olfactory bulb. The periventricular region (PVR) was then microdissected from each section and transferred to neurosphere and N-CFCA culture conditions. The absolute number of neurospheres, Colonies and LRCs in each region were counted and plotted according to their rostral-caudal distribution. Results: The NSA, N-CFCA, and LRC approach each detected precursor cells throughout the ventricular neuraxis, however, the number of precursors detected in individual 400μm sections varied from a minimum of 8 to a maximum of 891 depending upon the rostral-caudal coordinate assayed. Conclusion: By employing the N-CFCA to enumerate NSC-derived colonies we now report that the Majority of Endogenous Neural stem cells reside in the most rostral part of lateral ventricle in the adult mouse brain.

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