تولید بهینه عصاره توموری از سلول های گلیال B92

عنوان انگليسی

Optimized Production of Tumor Cell Lysates from B92 Glial cells

خلاصه انگلیسی

Introduction: Lysates from tumor cells are reported to induce maturation of dendritic cells (DCs) and are used in clinical settings for DC-based vaccination against tumors. Further studies showed that tumor lysates consist of HSP-peptide complexes (HSP-PC) that their expression increases in hyperthermia. This survey was designed to offer an efficient procedure for inducing the maximum amount of glial tumor cell lysates. Materials and Methods: Tumor cell line, B92 was cultured in RPMI supplemented with 10% FBS.To apply heat stress, culture flasks were sealed and immersed for 90 min in a water bath at 43°C, allowed to recover at 37°C for 8 h in a 5% CO 2 incubator. Then, cells were washed in condition medium and subjected to four freeze and thaw cycles to obtain a crude lysate. Results: Protein determination by the Bradford method showed a high level of the protein concentration in tumor lysate after exposure to heat. Conclusion: The present study proposes a valid method for generating of B92 rat tumor cell lysates consist of HSP-PCfrom cancer cells that form effective bases for immunotherapy approaches with unique properties.

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