سلیمی, احمد ، رحماندوست, ملینا ، پوراحمد, جلال (1395) ارزیابی سمیت 6 مرکاپتوپورین با استفاده از تکنیک ACMS. Iranian Journal of Pharmaceutical Sciences ــ 12 (3). ص.ص.65-74. شاپا 1735-2444
متن کامل
|
متنی
- نسخه چاپ شده
429kB |
آدرس اینترنتی رسمی : http://www.ijps.ir/article_23842.html
عنوان انگليسی
Toxicity evaluation of 6-mercaptopurine using accelerated cytotoxicity mechanism screening (ACMS) techniques
خلاصه انگلیسی
6- Mercaptopurine (6-MP) is widely used in clinic as an immunosuppressive for treatment of acute lymphocytic leukemia, Crohn’s disease, and ulcerative colitis with documented unpredictable hepatotoxicity. The potential molecular cytotoxic mechanisms of 6-MP against isolated rat hepatocytes were searched in this study using ―Accelerated Cytotoxicity Mechanism Screening (ACMS)‖ techniques. The concentration of 6-MP required to cause 50% cytotoxicity in 2 hour at 37∘C was detected to be 400 µM. A significant increase in 6-MP induced cytotoxicity and reactive oxygen species (ROS) formation, % mitochondrial membrane potential (MMP), lysosomal damage were observed. The addition of chloroquine (lysosomotropic agent), L-carnitine (inhibitor of membrane permeability transition (MPT), Diphenyleneiodonium (DPI) as an inhibitor of production of superoxide, and H2O2 by mitochondria and Dimethyl sulfoxide (DMSO) as a radical scavenger decreased 6-MP-induced cytotoxicity, ROS formation, collapse of MMP, and lysosomal damage. Results from this study suggest that 6-MP-induced cytotoxicity in isolated rat hepatocytes due to ROS formation, mitochondrial and lysosomal damages that resulted in crosstalk toxicity between mitochondrial and lysosomal damage and finally cell death.
نوع سند : | مقاله |
---|---|
زبان سند : | انگلیسی |
نویسنده اول : | احمد سلیمی |
نویسنده : | ملینا رحماندوست |
نویسنده مسئول : | جلال پوراحمد |
ضریب تاثیر و نمایه مجلات: | Indexed in: Scopus, Embase |
کلیدواژه ها (انگلیسی): | ACMS, Hepatotoxicity, lysosome, Mercaptopurine, Mitochondria, Reactive Oxygen Species. |
موضوعات : | QV فارماکولوژی > QV 600 سم شناسی |
بخش های دانشگاهی : | دانشکده داروسازی > بخش سم شناسی |
کد شناسایی : | 8511 |
ارائه شده توسط : | دکتر احمد سلیمی |
ارائه شده در تاریخ : | 01 اسفند 1395 08:28 |
آخرین تغییر : | 25 فروردین 1401 08:31 |
فقط پرسنل کتابخانه صفحه کنترل اسناد