جنیستئین از افزایش بیان ﻋﺎﻣﻞ ﻧﻮروﺗﺮوﻓﻴﻚ ﻣﺸﺘﻖ از ﻣﻐﺰدر هیپوکمپ موشهای فاقد تخمدان تشنجی جلوگیری می کند.

عنوان انگليسی

Genistein attenuates seizure-induced hippocampal brain-derived neurotrophic factor overexpressionin ovariectomized rats

خلاصه انگلیسی

Seizure activity not only promotes neuronal damage and cognitive dysfunction, but it also alters the levels of brain derived neurotrophic factor (BDNF) in various brain regions. Soy phytoestrogens (SPEs) have been shown to protect against seizure-induced adverse effects. Additionally, SPEs modulate the levels of BDNF in the brain. Thus, it is hypothesized that SPEs may also affect seizure-induced alterations of BDNF expression and ultrastructural damage in the hippocampus. We, therefore, examined the effects of genistein (one of the most important phytoestrogen) and soy extract containing genistein on seizure-induced hippocampal BDNF expression and ultrastructural damage in the hippocampal CA1 area of ovariectomized (OVX) rats. The OVX rats received an injection of genistein (0.5 or 5 mg/kg), soy extract (2 or 20 mg/kg), or vehicle during four consecutive days. An hour after the last injection, kainic acid (KA, 0.5 µg/1µl) or vehicle was perfused into the left lateral ventricle to induce generalized tonic-clonic seizures. A week later, the hippocampal BDNF expression and ultrastructural characteristics ofCA1 pyramidal neurons were examined. The generalized seizures induced BDNF mRNA and protein overexpression in the hippocampus and neuronal damage in the hippocampal CA1 area. Moreover, only genistein at the lower dose (0.5 mg/kg) and soy extract at the higher dose (20 mg/kg) attenuated the above-mentioned negative effects of the seizure activity; however, neither of them affected the seizure activity. The results suggest that SPEs may have a preventive effect against seizure-induced hippocampal BDNF mRNA and protein overexpression accompanied by a neuroprotective effect on ultrastructural damage in the hippocampal CA1 area of OVX rats.

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