محمودی نیا, سمیرا ، نیاپور, علی ، قاسمی حمیدآبادی, هاتف ، مأذنی, محمد (1398) 2,4-D باعث القا استرس اکسیداتیو و اپوپتور در سلول های بنیادی پالپ دندان می شود. Environmental Science and Pollution Research ــ 26 (25). ص.ص.26170-26183. شاپا 0944-1344
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آدرس اینترنتی رسمی : https://link.springer.com/article/10.1007/s11356-0...
عنوان انگليسی
2,4-D causes oxidative stress induction and apoptosis in human dental pulp stem cells (hDPSCs)
خلاصه انگلیسی
2,4-Dicholorophenoxy acetic acid (2,4-D) is a worldwide used hormone herbicide. Human dental pulp stem cells (hDPSCs) as a potential source of mesenchymal stem cells provide a confident model system for the assessments of chemicals in vitro. The main objective of this study was to examine the biological effects and damages attributed to 2,4-D on hDPSCs. hDPSCs were isolated from third molar pulp tissues and their mesenchymal identity were evaluated. Then, hDPSCs were treated with increasing concentrations of 2,4-D (0.1 μM-10 mM). Cell viability assay and cumulative cell counting were carried out to address 2,4-D effects on biological parameters of hDPSCs. Cell cycle distribution, ROS level and ALP activity were measured before and after treatment. AO/EB staining and caspase 3/7 activity were investigated to detect the possible mechanisms of cell death. Flow-cytometric immunophenotyping and differentiation data confirmed the mesenchymal identity of cultivated hDPSCs. 2,4-D treatment caused a hormetic response in the viability and growth rate of hDPSCs. G0/G1 cell cycle arrest, enhanced ROS level, and reduced ALP activity were detected in hDPSCs treated with EC50 dose of 2,4-D. AO/EB staining showed a higher percentage of alive cells in lower concentrations of the herbicide. The increment in 2,4-D dose and the number of early and late apoptotic cells were increased. DAPI staining and caspase 3/7 assay validated the induction of apoptosis. 2,4-D concentrations up to 100 μM did not affect hDPSCs viability and proliferation. The intense cellular oxidative stress and apoptosis were observed at higher concentration.
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده اول : | سمیرا محمودی نیا |
نویسنده مسئول : | علی نیاپور |
نویسنده : | هاتف قاسمی حمیدآبادی |
نویسنده : | محمد مأذنی |
ضریب تاثیر و نمایه مجلات: | IF:4.223 Indexing in: ISI, PubMed/Medline, Scopus |
کلیدواژه ها (انگلیسی): | Mesenchymal stromal cells, hDPSCs, 2,4-D, Viability, Hormesis, Apoptosis |
موضوعات : | QV فارماکولوژی > QV 600 سم شناسی QU بیوشیمی |
بخش های دانشگاهی : | دانشكده پزشكي > گروه علوم پایه > بخش بیوشیمی دانشكده پزشكي > گروه علوم پایه > بخش علوم تشريح |
کد شناسایی : | 12041 |
ارائه شده توسط : | دکتر علی نیاپور |
ارائه شده در تاریخ : | 27 تیر 1398 10:29 |
آخرین تغییر : | 29 مرداد 1401 10:02 |
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