اصلانی, محمدرضا ، قبادی مراللو, حسن ، پناهپور, حمداله ، احمدی, مهدی ، خاکسار, مجید ، حیدرزاده, مرتضی (1398) تغییرات بیان ژنهای شبکه آندوپلاسمی و سطوح پروتئین NF-kB بافت ریه در رتهای نر و ماده حساس شده با اوآلبومین. Life sciences ــ 247 (April). ص. 2020. شاپا 0024-3205
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آدرس اینترنتی رسمی : https://www.sciencedirect.com/science/article/pii/...
عنوان انگليسی
Modification of lung endoplasmic reticulum genes expression and NF-kB protein levels in obese ovalbumin-sensitized male and female rats
خلاصه انگلیسی
Aims: Previous studies showed a close relationship between obesity and asthma. In this study, we investigated the expression of endoplasmic reticulum (ER) stress genes in the lung tissue of obese ovalbumin (OVA)-sensitized male and female rats. Main methods: The rats were divided into eight groups (n = 5 per group) as follows: female and male rats fed with normal diet (FND and MND, respectively), female and male OVA-sensitized rats fed with normal diet (FOND and M-OND, respectively), female and male rats fed with high-fat diet (F-HFD and M-HFD, respectively), female and male OVA-sensitized rats fed with high-fat diet (F-OHFD and M-OHFD, respectively). All rats were fed with a high-fat diet or standard pelts for 8 weeks, and for another 4 weeks, they were sensitized by OVA or saline. At the end of the study, lung tissue NF-kB protein level was assessed, and ER stress markers genes expression was determined by Real Time-PCR. Key finding: OVA-sensitization and diet-induced obesity caused the curve of methacholine concentration-response to shift to the left. In addition, the results indicated that the EC50 (the effective concentration of methacholine generating 50% of peak response) in F-OHFD rats was statistically lower than that of the M-OHFD group (p < 0.05). Moreover, the results showed that diet-induced obesity increased the expression of ATF4, ATF6, GRP78, XBP-1, and CHOP as well as the protein level of NF-kB in this experimental model of asthma, markedly in the F-OHFD group. Significance: The results suggest that ER stress may be involved in the pathogenesis of asthma observed in obese OVA-sensitized rats, especially in the female animals.
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده مسئول : | محمدرضا اصلانی |
نویسنده : | حسن قبادی مراللو |
نویسنده : | حمداله پناهپور |
نویسنده : | مهدی احمدی |
نویسنده : | مجید خاکسار |
نویسنده : | مرتضی حیدرزاده |
ضریب تاثیر و نمایه مجلات: | IF: 3.448 Indexed in: ISI, Scopus, PubMed/Medline, Embase |
کلیدواژه ها (انگلیسی): | Asthma, Obesity, Endoplasmic reticulum stress , GRP78, CHOP |
موضوعات : | QT فیزیولوژی WF سیستم تنفسی |
بخش های دانشگاهی : | دانشكده پزشكي > گروه داخلی ، قلب ، عفونی دانشكده پزشكي > گروه علوم پایه > بخش میکروبیولوژی |
کد شناسایی : | 12741 |
ارائه شده توسط : | دکتر محمد رضا اصلانی |
ارائه شده در تاریخ : | 19 اسفند 1398 12:52 |
آخرین تغییر : | 11 تیر 1399 08:44 |
فقط پرسنل کتابخانه صفحه کنترل اسناد